Flow cytometry analysis of rat kidney
WebMar 25, 2015 · Using flow cytometry, immunofluorescence, and RT-PCR, we show that our method is suitable for studying and isolating proximal tubular cells with an anti Prominin … WebOct 12, 2024 · Renal fibrosis represents the final common outcome of chronic kidney disease of virtually any etiology. However, the mechanism underlying the evolution of renal fibrosis remains to be addressed. This study sought to clarify whether RIP1-RIP3-mediated necroptosis is involved in renal fibrosis via Wnt3α/β-catenin/GSK-3β signaling in vitro …
Flow cytometry analysis of rat kidney
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WebBlood, lungs and kidneys were collected 24 hours post-surgery. Sepsis-induced changes in platelet activation, secretion and platelet interaction with immune cells were measured by flow... WebFeb 10, 2024 · Flow cytometry staining was performed as previously described using specific antibody panels for myeloid cells in rat tissue, which included CD45 (clone OX-1), CD11b/c (clone OX-42), and the granulocyte marker RP-1 [99,100,101] comparable to Ly6g in mouse. These antibodies are equivalent to mouse tissue used in rats for myeloid …
WebOct 18, 2016 · October 18th, 2016. This manuscript describes a detailed protocol for phenotypic and quantitative analysis of resident macrophages from rat kidneys by flow cytometry. The resulting stained cells can be also used for other applications, including cell sorting, gene expression analysis or functional studies, thus increasing the information ... WebNeuritin was discovered in the rat as a neuropeptide that is involved in neuronal plasticity [1,2,3]. ... Positive supernatants were further analyzed by flow cytometry analysis and selected hybridoma cells were subcloned twice by limiting dilution to obtain stable monoclonal cell lines. Experiments in this work were performed with the hybridoma ...
WebOct 25, 2015 · Intracellular flow cytometry. For flow cytometry analysis, differentiated cells were fixed and stained using the Cytofix/Cytoperm Kit (BD) following the manufacturer's instructions. ... Blood Institute (NHLBI) [U01HL100408, U01HL107440 and R21HL120781] and National Institute of Diabetes and Digestive and Kidney Diseases … WebFlow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/50 followed by goat anti rat IgG FITC secondary antibody at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.
WebFeb 17, 2024 · Flow cytometry analysis reported low cell mortality during the isolation protocol. There are several factors that may affect cell viability and surface markers …
WebTeknologi flow cytometer dapat dengan cepat menganalisis sebuah sel saat dialirkan dalam larutan khusus dan melalui laser tunggal atau ganda. Metode ini digunakan untuk … phil lehr\\u0027s steakery san franciscoWebOct 1, 1992 · Analysis and isolation of renal tubular cells by flow cytometry. The cells of the renal cortex have rich heterogeneity of structure and function. Flow cytometry, the … philleigh fayreWebPrevious studies have shown that gossypol induces cell death in cancer cells [18,19] and germline stem cells ; hence we used primary neonatal rat cardiomyocytes cultured in vitro and treated with GAA (20 and 50 μM) to assess the induction of cell death via flow cytometry analysis with Annexin V-FITC and PI for apoptosis and necrosis detection ... phill elstonWebJan 14, 2016 · The rat is a commonly used model for immunological investigation. Yet basic research and characterisation of leukocyte populations and sub-sets lags far behind murine research, with inconsistency on reported leukocyte markers and their overlap. These shortcomings limit the opportunity for more complex and advanced rat immunology … trying or distressing experienceWebOct 24, 2024 · Podocyte hypertrophic stress and detachment precedes hyperglycemia or albuminuria in a rat model of obesity and type2 diabetes-associated nephropathy ... based on flow-cytometry analysis of ... philleigh fairWebApr 10, 2024 · Flow cytometry analysis to assess transfection efficiency. To demonstrate transfection efficiency, flow cytometry (Beckman Cytoflex) was performed. ... we assessed the in vivo toxicity of mRNA-cLNP. After tissue repair was completed at 8 weeks, hearts, livers, lungs, and kidneys of three rats in each group (NC, mRNA, mRNA-LNP, mRNA … phil lehr\u0027s steakery san franciscoWebA flow cytometry method was used to characterize lymphoid tissue-associated lymphocyte subsets in the Sprague-Dawley rat. Mononuclear leukocytes from peripheral blood, spleen, and thymus of male and female rats were labeled with a panel of fluorescently tagged monoclonal and polyclonal antibodies directed against specific cell-surface proteins. phil leighton sheffield